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Antigen Access Solutions

HIER Buffers

HIER buffers unmask epitopes that have been cross-linked by formalin fixation. These solutions are specially formulated for superior pH stability at high temperatures and will help prevent the loss of pH sensitive antigens. All Access solutions incorporate Assure™ colour-coded pH indicator technology that allows with visual inspection to verify that the solution is at the correct dilution and pH. With the exception of EDTA all products can be stored at room temperature and all buffers are sodium azide and thimerosal free. These products are specially formulated for superior pH stability at high temperature, and will help prevent the loss of pH-sensitive antigens. The Access solutions also block endogenous peroxidase and reduce non-specific background, except Access Citrate, Access Tris and Access EDTA.

Name  Description  Product code 
Access Supreme  Eliminates the need for multiple buffers including citrate, EDTA or high pH Tris. Antibody titres are doubled and tripled when compared to citrate buffer. Access Supreme is non-toxic, non-flammable and odorless, pH 6.2. MP-600 
Access Rodent  A Novel Heat-Induced Epitope Retrieval (HIER) Solution for Rodent Tissues. Used for the unmasking of epitopes cross-linked by formalin fixation and blocking endogenous mouse and rat IgG. It has been specially formulated for use on mouse and rat tissues with emphasis on rat brain. Access Rodent has been formulated to work with the MenaPath Promark series of mouse, rat, goat and rabbit polymer detection kits. It is designed for research use only, and not intended for therapeutic and diagnostic purposes.  MP-913
Access Revelation  Access Revelation is a specially formulated heat-retrieval solution. It is used in conjunction with the Antigen Access Unit. Access Revelation is non-toxic, non-flammable and odourless. Antibody titres are increased compared to citrate buffer, pH 6.0, and background staining is reduced significantly. Access Revelation also blocks endogenous peroxidase and mercury crystals.  MP-607
Access Super Access Super is a Tris-Based formulation, pH 9.5, and is non-toxic, non-flammable and odourless. Tissues can be deparaffinised and then slides can be placed into the Access Super used in conjunction with the Antigen Access Unit or other heating methods. In most cases, antibody titres will increase compared to other heat-retrieval buffers.  MP-606
Access EDTA  An EDTA buffer formulation with preservative. This product is specially formulated for superior pH stability at high temperature, and will help prevent the loss of pH-sensitive antigens (pH range of 8.5 after dilution).  MP-605
Access Citrate  A specially formulated citrate buffer, with preservative, designed for superior pH stability at high-temperature incubations. The pH 6.0 formulation helps prevent the loss of pH-sensitive antigens.  MP-603
Access Tris This is a Tris buffer formulation, pH 9.5 with preservative. Specially formulated for superior pH stability at high temperature, this product will help prevent the loss of pH-sensitive antigens.  MP-604

Deparaffinisation

Access Revelation and Access Super HIER solutions may be used in a two-step deparaffinisation and antigen retrieval in combination with Slide Wash.

Name  Description  Product code 
Slide Wash (post-HMAR) Clarifying reagent to be used with Access Revelation or Access Super HIER reagents.  MP-621
Dewax Solution  Dewax Solution is a water-soluble deparaffinisation reagent which can be used for IHC, H&Es, and special stains. Paraffin is completely dissolved by this non-flammable and non-hazardous, safe-to-use solution. Eliminates the use of xylenes and alcohols, reducing disposal costs and toxic waste. Dewax Solution can be used with a two-step deparaffinisation retrieval protocol using Access Revelation or Access Super HIER reagents.  MP-622
Slide Wash Slide Wash is a non-hazardous alternative to xylene for the deparaffinisation and clearing of tissue sections. The flash point for this reagent is above 60°C, double that of xylene. Slide Wash has been designated non-hazardous on the basis of aquatic toxicity, and requires no fume hood or ventilation. MP-SLDWSH-PG1

Enzymes

Pretreatment with an enzyme digestion is required for many IHC protocols. The enzyme digestion kits have been designed to provide the optimum digestion and are optimised for ease of use.

Name  Product code 
Trypsin Enzyme Digestion Kit   MP-955
Pepsin Enzyme Digestion Kit  MP-956
Pronase Enzyme Digestion Kit  MP-957
Protease XXIV Kit  MP-960