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Blocking Reagents

Endogenous Peroxidase Blockers

Peroxide Block (MP-968)

A potent blocker for endogenous peroxidase, optimised for use with paraffin-embedded sections. The product is highly stable and superior to 3% hydrogen peroxide in water and methanol. This product can be stored at room temperature or refrigerated. The specially formulated diluent used makes the blocker compatible for both paraffin and frozen sections.

Peroxide Block Non-H202 (MP-969-PXA)

A non-hydrogen peroxide-based formula that is safe and extremely gentle on tissues and cells. This novel formula is highly effective in quenching endogenous peroxidase. It is extremely effective if used after antigen retrieval methods in paraffin-embedded, formalin-fixed tissues, or can be safely used for frozen sections.

Non-specific Background

Protein Block (MP-965)

A blue colour-coded PBS Solution of goat serum and Tween 20. It is designed to block non-specific background staining in IHC procedures and is formulated to work in five minutes.

Universal Background Blocker (MP-974)

Casein has proven to be a superior blocking reagent as compared to serum proteins. Universal Background Blocker is specifically formulated for superior pH stability and is sodium azide and thimerosal free. It can also be used with a semi-automated or manual staining protocol.

Background Blocker with Casein (MP-966)

A blue colour-coded universal blocking reagent for IHC procedures. It has been formulated with recombinant blocking proteins and is serum free. It can be used with any detection system and on both human and animal tissues and for cell culture techniques.

Super Background Blocker (MP-967)

A goat serum with surfactant blocker that is used when excessive background problems exist. Designed for use with X-Cell Plus Multiplex detection (MP-XLCT523/ MP-XLCT525), not suitable for two-step detections or intelliPATH™ detection.

Endogenous Biotin

Avidin Biotin Blocking Kit (MP-972)

The Avidin and Biotin solutions are colour-coded with a pH indicator. In most cases, endogenous biotin in tissue sections is masked by formalin fixation. However, if avidin-biotin IHC detection systems are used with frozen sections or tissues pretreated with a heat-induced, epitope retrieval method (HIER), an avidin-biotin blocking technique may be needed. It can also be used on an automated staining system.

Mouse IgG Block (MP-975)

Mouse IgG Block is an immunohistochemical reagent designed for blocking endogenous mouse IgG in mouse tissues and red blood cells. After the application of mouse detective, a mouse monoclonal antibody can be applied followed by a biotinylated mouse link and Streptavidin label used for detection of mouse on mouse. It can also be used on an automated staining system.