inner header

Chromogens

product pic

Key product features

  • Used with the two most commonly used enzyme labels
  • Produces a coloured end-product that can be visualised under a brightfield microscopy

Product Overview

Product overview

Menarini Diagnostics offers the largest variety of IHC-specific permanent chromogens, for use with the two most commonly used enzyme labels: Horseradish Peroxidase (HRP) and Alkaline Phosphatase (AP). Chromogens are chemical compounds used within IHC staining procedures that produce a coloured end-product that can be visualised under brightfield microscopy.

HRP

Chromogens Compatible with HRP

Product name

Product Code

Liquid Stable DAB Chromogen Kit MP-860

Product Summary

 

hrp 1 cropLiquid Stable DAB is superior to conventional and Liquid DAB in terms of stability and staining intensity. This chromogen is compatible with horseradish peroxidase (HRP), not soluble in alcohol and xylene, and can be coverslipped just like any other DAB. When in the presence of peroxidase enzyme, DAB produces a brown stain. Liquid Stable DAB may increase antibody titers by two fold; and can be used manually or on automated stainers.

Product name

Product Code

Liquid DAB Chromogen Kit MP-859

Product Summary

 

DAB is widely used in IHC staining and immunoblotting and produces a brown precipitate that is hrp 2 cropinsoluble in alcohol and xylene for permanent mounting. The kit consists of three components, liquid stable DAB chromogen, DAB substrate buffer and DAB enhancer. The enhancer adds contrast and increases the staining intensity.

Product name

Product Code

AEC Chromogen Kit MP-810

Product Summary

 

hrp 3 crop

AEC is a widely used chromogen for immunohistochemical procedures. AEC produces a reddish-orange stain. However, it is not soluble in alcohol or xylene and can be coverslipped just like DAB and it does not fade with permanent mounting media. When in the presence of a peroxidase enzyme, AEC produces a reddish-orange stain. This product comes in a 4-component system consisting of ready-to-use buffer, stabiliser, chromogen and hydrogen peroxide. This system is compatible with both manual and automatic coverslippers.

Product name

Product Code

Purple Chromogen Kit MP-811

Product Summary

 

hrp 4 crop

The Purple Chromogen Kit is a permanent chromogen substrate that is insoluble in alcohol and xylene, and can be coverslipped just like DAB. In the presence of a peroxidase enzyme, MenaPath Purple produces a permanent lavender-purple stain. The kit consists of four components, ready-to-use buffer, stabiliser, chromogen and hydrogen peroxide, MenaPath Purple may also be used in double- and triple-stain procedures, nitrocellulose blots, and can be viewed by both brightfield and darkfield microscopy.

Product name

Product Code

Green Chromogen Kit MP-807

Product Summary

 
MenaPath Green is a novel permanent chromogen that produces a green stain in the presence of hrp 5 crophorseradish peroxidase (HRP). The kit consists of liquid Green chromogen and buffer that are stable, when mixed, for at least 4 hours at room temperature. MenaPath Green is clearly distinguishable from Ultimate Red and DAB on a single slide, enabling high flexibility for its application in Multiplex IHC. Developed for both manual and automated platforms, MenaPath Green is suitable for both immunohistochemistry (IHC) and in situ hybridisation (ISH) applications such as HPV, CMV, EBV, p63, TTF-1, Ki-67 and other targets antigens such as blood and lymphatic vessels, and basal and myoepithelial cells.

Product name

Product Code

Black Chromogen Kit MP-815

Product Summary

 
MenaPath Black is a novel permanent chromogen that produces a dark grey to black stain in the hrp 6 croppresence of horseradish peroxidase (HRP). The kit consists of liquid black chromogen and buffer that is stable for 8 hours at room temperature once mixed. MenaPath Black is clearly distinguishable from Ultimate Red, DAB, MenaPath Green and MenaPath Blue on a single slide, enabling high flexibility for Multiplex IHC applications. Developed for both manual and automated platforms, MenaPath Black is suitable for both immunohistochemistry (IHC) and in situ hybridisation (ISH) applications such as HPV, CMV, EBV, Kappa, Lambda, p63, HMWCK, TTF-1, Ki-67, and other targets/antigens such as cytokeratins, melanoma, and basal and myoepithelial cells.

DAB Enhancing Products

Adds contrast and staining intensity

Product Name

Product Code

DAB Enhancer MP-859-EN6
DAB Post-Enhancing Solution MP-DS830

AP

Chromogen compatible with Alkaline Phosphatase (AP)

Product Name

Product Code

Ultimate Red Chromogen Kit MP-806

Product Summary

 

AP 1 cropUltimate Red is a faster, sharper and more stable red chromogen. The newly formulated Ultimate Red is quick and sensitive, similar to DAB, providing an accelerated protocol and consistent staining quality. The sharp and intense red stain creates superior contrast for Multiplex IHC™. These advanced features of Ultimate Red result in a faster turnaround time, greater staining consistency and improved flexibility.

Product name

Product Code

Fast Red Chromogen Kit MP-FR805

Product Summary

 

AP 2 crop

Fast Red is a widely used chromogen for immunohistochemical staining. When in the presence of alkaline phosphatase (AP) enzyme, Fast Red produces a bright fuchsin-red precipitate that is insoluble in organic solvents and can be coverslipped with a permanent mounting media. This product is available in a stable two-component system, consisting of Fast Red Chromogen and Buffer. 

Product name

Product Code

Blue Chromogen Kit MP-813

Product Summary

 
MenaPath Blue can be coverslipped with a non xylene or toluene-based permanent mounting media. AP 3 cropThis chromogen produces a bright royal blue precipitate in the presence of alkaline phosphatase enzyme. Not for use with PBS.

  

Product Enquiries

product enquiriesContact our product sales team today

For more information or to request a demonstration please contact us today by calling 0118 944 4100 or emailing us on enquiries@menarinidiag.co.uk