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Antigen Access Solutions

HIER Buffers

HIER buffers unmask epitopes that have been cross-linked by formalin fixation. These solutions are specially formulated for superior pH stability at high temperatures and will help prevent the loss of pH sensitive antigens. All Access solutions incorporate Assure™ colour-coded pH indicator technology that allows with visual inspection to verify that the solution is at the correct dilution and pH. With the exception of EDTA all products can be stored at room temperature and all buffers are sodium azide and thimerosal free. These products are specially formulated for superior pH stability at high temperature, and will help prevent the loss of pH-sensitive antigens. The Access solutions also block endogenous peroxidase and reduce non-specific background, except Access Citrate, Access Tris and Access EDTA.

Name  Product code Description  Old Name Old Product code 
Diva Decloaker  DV2004 Eliminates the need for multiple buffers including citrate, EDTA or high pH Tris. Antibody titres are doubled and tripled when compared to citrate buffer. Access Supreme is non-toxic, non-flammable and odorless, pH 6.2. Access Supreme MP-600 
Rodent Decloaker RD913 A Novel Heat-Induced Epitope Retrieval (HIER) Solution for Rodent Tissues. Used for the unmasking of epitopes cross-linked by formalin fixation and blocking endogenous mouse and rat IgG. It has been specially formulated for use on mouse and rat tissues with emphasis on rat brain. Access Rodent has been formulated to work with the Biocare Promark series of mouse, rat, goat and rabbit polymer detection kits. It is designed for research use only, and not intended for therapeutic and diagnostic purposes. Access  MP-913
Reveal Decloaker RV1000 Access Revelation is a specially formulated heat-retrieval solution. It is used in conjunction with the Antigen Access Unit. Access Revelation is non-toxic, non-flammable and odourless. Antibody titres are increased compared to citrate buffer, pH 6.0, and background staining is reduced significantly. Access Revelation also blocks endogenous peroxidase and mercury crystals. Access Revelation  MP-607
Borg Decloaker BD1000 Access Super is a Tris-Based formulation, pH 9.5, and is non-toxic, non-flammable and odourless. Tissues can be deparaffinised and then slides can be placed into the Access Super used in conjunction with the Antigen Access Unit or other heating methods. In most cases, antibody titres will increase compared to other heat-retrieval buffers. Access Super  MP-606
EDTA decloaker CB917 An EDTA buffer formulation with preservative. This product is specially formulated for superior pH stability at high temperature, and will help prevent the loss of pH-sensitive antigens (pH range of 8.5 after dilution). Access EDTA  MP-605
Antigen Decloaker CB910 A specially formulated citrate buffer, with preservative, designed for superior pH stability at high-temperature incubations. The pH 6.0 formulation helps prevent the loss of pH-sensitive antigens. Access Citrate  MP-603
Nuclear Decloaker CB911 This is a Tris buffer formulation, pH 9.5 with preservative. Specially formulated for superior pH stability at high temperature, this product will help prevent the loss of pH-sensitive antigens. Access Tris  MP-604

Deparaffinisation

Reveal Decloaker and Borg Decloaker HIER solutions may be used in a two-step deparaffinisation and antigen retrieval in combination with Slide Wash.

Name  Code Description  Old Product name  Old Product code 
Hot rinse HTR100 Clarifying reagent to be used with Access Revelation or Access Super HIER reagents. Slide Wash (post-HMAR)  MP-621
Aqua DePar  ADP100 Dewax Solution is a water-soluble deparaffinisation reagent which can be used for IHC, H&Es, and special stains. Paraffin is completely dissolved by this non-flammable and non-hazardous, safe-to-use solution. Eliminates the use of xylenes and alcohols, reducing disposal costs and toxic waste. Dewax Solution can be used with a two-step deparaffinisation retrieval protocol using Access Revelation or Access Super HIER reagents. Dewax Solution  MP-622

Enzymes

Pretreatment with an enzyme digestion is required for many IHC protocols. The enzyme digestion kits have been designed to provide the optimum digestion and are optimised for ease of use.

Name  Product code Old Name  Old Product code 
Carezyme I Trypsin TRP955 Trypsin Enzyme Digestion Kit   MP-955
Carezyme2 Pepsin PEP956 Pepsin Enzyme Digestion Kit  MP-956
Pronase Kit PRT957 Pronase Enzyme Digestion Kit  MP-957
Protease PR960 Protease XXIV Kit  MP-960

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