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Chromogens

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Key product features

  • Used with the two most commonly used enzyme labels
  • Produces a coloured end-product that can be visualised under a brightfield microscopy

Product Overview

Product overview

Menarini Diagnostics offers the largest variety of IHC-specific permanent chromogens, for use with the two most commonly used enzyme labels: Horseradish Peroxidase (HRP) and Alkaline Phosphatase (AP). Chromogens are chemical compounds used within IHC staining procedures that produce a coloured end-product that can be visualised under brightfield microscopy.

HRP

Chromogens Compatible with HRP

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 Betazoid DAB BDB2004 Liquid Stable DAB Chromogen Kit MP-860

Product Summary

 

 

 

hrp 1 cropBetazoid DAB is superior to conventional DAB in terms of stability and staining intensity. This chromogen is compatible with horseradish peroxidase (HRP), not soluble in alcohol and xylene, and can be coverslipped just like any other DAB. When in the presence of peroxidase enzyme, DAB produces a brown stain. Betazoid DAB may increase antibody titers by two fold; and can be used manually or on automated stainers.

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Cardassian DAB DBC859 Liquid DAB Chromogen Kit MP-859

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DAB is widely used in IHC staining and immunoblotting and produces a brown precipitate that is hrp 2 cropinsoluble in alcohol and xylene for permanent mounting. The kit consists of three components, liquid stable DAB chromogen, DAB substrate buffer and DAB enhancer. The enhancer adds contrast and increases the staining intensity.

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Romulin AEC RAEC810 AEC Chromogen Kit MP-810

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Romulin AEC is a widely used chromogen for immunohistochemical procedures. AEC produces a reddish-orange stain. However, it is not soluble in alcohol or xylene and can be coverslipped just like DAB and it does not fade with permanent mounting media. When in the presence of a peroxidase enzyme, AEC produces a reddish-orange stain. This product comes in a 4-component system consisting of ready-to-use buffer, stabiliser, chromogen and hydrogen peroxide. This system is compatible with both manual and automatic coverslippers.

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Bajoran Purple Permanent Chrom BJP811 Purple Chromogen Kit MP-811

Product Summary

     

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The Bajoran Purple permanent Chrom is a permanent chromogen substrate that is insoluble in alcohol and xylene, and can be coverslipped just like DAB. In the presence of a peroxidase enzyme, Bajoran Purple produces a permanent lavender-purple stain. The kit consists of four components, ready-to-use buffer, stabiliser, chromogen and hydrogen peroxide, Bajoran Purple may also be used in double- and triple-stain procedures, nitrocellulose blots, and can be viewed by both brightfield and darkfield microscopy.

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Vina Green™ Chromogen Kit VG807 Green Chromogen Kit MP-807

Product Summary

     
Vina Green is a novel permanent chromogen that produces a green stain in the presence of hrp 5 crophorseradish peroxidase (HRP). The kit consists of liquid Green chromogen and buffer that are stable, when mixed, for at least 4 hours at room temperature. Vina Green is clearly distinguishable from Warp Red and DAB on a single slide, enabling high flexibility for its application in Multiplex IHC. Developed for both manual and automated platforms, Vina Green is suitable for both immunohistochemistry (IHC) and in situ hybridisation (ISH) applications such as HPV, CMV, EBV, p63, TTF-1, Ki-67 and other targets antigens such as blood and lymphatic vessels, and basal and myoepithelial cells.

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Deep Space Black TM Chromogen Kit BRI4015 Black Chromogen Kit MP-815

Product Summary

     
Deep space Black is a novel permanent chromogen that produces a dark grey to black stain in the hrp 6 croppresence of horseradish peroxidase (HRP). The kit consists of liquid black chromogen and buffer that is stable for 8 hours at room temperature once mixed. Deep space Black is clearly distinguishable from Warp Red, DAB, Vina Green and Ferangi Blue on a single slide, enabling high flexibility for Multiplex IHC applications. Developed for both manual and automated platforms, Deep Space Black is suitable for both immunohistochemistry (IHC) and in situ hybridisation (ISH) applications such as HPV, CMV, EBV, Kappa, Lambda, p63, HMWCK, TTF-1, Ki-67, and other targets/antigens such as cytokeratins, melanoma, and basal and myoepithelial cells.

DAB Enhancing Products

Adds contrast and staining intensity

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DAB Sparkle DS830  DAB Post-Enhancing Solution  MP-DS830

AP

Chromogen compatible with Alkaline Phosphatase (AP)

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Warp Red Chromogen Kit WR-806 Ultimate Red Chromogen Kit MP-806

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AP 1 cropWarp Red is a faster, sharper and more stable red chromogen. The newly formulated Warp Red is quick and sensitive, similar to DAB, providing an accelerated protocol and consistent staining quality. The sharp and intense red stain creates superior contrast for Multiplex IHC™. These advanced features of Warp Red result in a faster turnaround time, greater staining consistency and improved flexibility.

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Vulcan Fast Red Kit 2 FR805 Fast Red Chromogen Kit MP-FR805

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Vulcan Fast Red is a widely used chromogen for immunohistochemical staining. When in the presence of alkaline phosphatase (AP) enzyme, Vulcan Fast Red produces a bright fuchsin-red precipitate that is insoluble in organic solvents and can be coverslipped with a permanent mounting media. This product is available in a stable two-component system, consisting of Vulcan Fast Red Chromogen and Buffer. 

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Ferangi Blue™ Chromogen Kit 2 FB813 Blue Chromogen Kit MP-813

Product Summary

     
Ferangi Blue™ can be coverslipped with a non xylene or toluene-based permanent mounting media. AP 3 cropThis chromogen produces a bright royal blue precipitate in the presence of alkaline phosphatase enzyme. Not for use with PBS.

  

Product Enquiries

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For more information or to request a demonstration please contact us today by calling 0118 944 4100 or emailing us on enquiries@menarinidiag.co.uk