Chromogens
Key product features
- Used with the two most commonly used enzyme labels
- Produces a coloured end-product that can be visualised under a brightfield microscopy
Product Overview
Product overview
Menarini Diagnostics offers the largest variety of IHC-specific permanent chromogens, for use with the two most commonly used enzyme labels: Horseradish Peroxidase (HRP) and Alkaline Phosphatase (AP). Chromogens are chemical compounds used within IHC staining procedures that produce a coloured end-product that can be visualised under brightfield microscopy.
HRP
Chromogens Compatible with HRP
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| Betazoid DAB | BDB2004 | Liquid Stable DAB Chromogen Kit | MP-860 |
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Betazoid DAB is superior to conventional DAB in terms of stability and staining intensity. This chromogen is compatible with horseradish peroxidase (HRP), not soluble in alcohol and xylene, and can be coverslipped just like any other DAB. When in the presence of peroxidase enzyme, DAB produces a brown stain. Betazoid DAB may increase antibody titers by two fold; and can be used manually or on automated stainers.
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| Cardassian DAB | DBC859 | Liquid DAB Chromogen Kit | MP-859 |
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DAB is widely used in IHC staining and immunoblotting and produces a brown precipitate that is |
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insoluble in alcohol and xylene for permanent mounting. The kit consists of three components, liquid stable DAB chromogen, DAB substrate buffer and DAB enhancer. The enhancer adds contrast and increases the staining intensity.
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| Romulin AEC | RAEC810 | AEC Chromogen Kit | MP-810 |
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Romulin AEC is a widely used chromogen for immunohistochemical procedures. AEC produces a reddish-orange stain. However, it is not soluble in alcohol or xylene and can be coverslipped just like DAB and it does not fade with permanent mounting media. When in the presence of a peroxidase enzyme, AEC produces a reddish-orange stain. This product comes in a 4-component system consisting of ready-to-use buffer, stabiliser, chromogen and hydrogen peroxide. This system is compatible with both manual and automatic coverslippers. |
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| Bajoran Purple Permanent Chrom | BJP811 | Purple Chromogen Kit | MP-811 |
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The Bajoran Purple permanent Chrom is a permanent chromogen substrate that is insoluble in alcohol and xylene, and can be coverslipped just like DAB. In the presence of a peroxidase enzyme, Bajoran Purple produces a permanent lavender-purple stain. The kit consists of four components, ready-to-use buffer, stabiliser, chromogen and hydrogen peroxide, Bajoran Purple may also be used in double- and triple-stain procedures, nitrocellulose blots, and can be viewed by both brightfield and darkfield microscopy. |
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| Vina Green™ Chromogen Kit | VG807 | Green Chromogen Kit | MP-807 |
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Vina Green is a novel permanent chromogen that produces a green stain in the presence of  horseradish peroxidase (HRP). The kit consists of liquid Green chromogen and buffer that are stable, when mixed, for at least 4 hours at room temperature. Vina Green is clearly distinguishable from Warp Red and DAB on a single slide, enabling high flexibility for its application in Multiplex IHC. Developed for both manual and automated platforms, Vina Green is suitable for both immunohistochemistry (IHC) and in situ hybridisation (ISH) applications such as HPV, CMV, EBV, p63, TTF-1, Ki-67 and other targets antigens such as blood and lymphatic vessels, and basal and myoepithelial cells. |
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| Deep Space Black TM Chromogen Kit | BRI4015 | Black Chromogen Kit | MP-815 |
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Deep space Black is a novel permanent chromogen that produces a dark grey to black stain in the  presence of horseradish peroxidase (HRP). The kit consists of liquid black chromogen and buffer that is stable for 8 hours at room temperature once mixed. Deep space Black is clearly distinguishable from Warp Red, DAB, Vina Green and Ferangi Blue on a single slide, enabling high flexibility for Multiplex IHC applications. Developed for both manual and automated platforms, Deep Space Black is suitable for both immunohistochemistry (IHC) and in situ hybridisation (ISH) applications such as HPV, CMV, EBV, Kappa, Lambda, p63, HMWCK, TTF-1, Ki-67, and other targets/antigens such as cytokeratins, melanoma, and basal and myoepithelial cells. |
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DAB Enhancing Products
Adds contrast and staining intensity
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| DAB Sparkle | DS830 | DAB Post-Enhancing Solution | MP-DS830 |
AP
Chromogen compatible with Alkaline Phosphatase (AP)
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| Warp Red Chromogen Kit | WR806 | Ultimate Red Chromogen Kit | MP-806 |
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Warp Red is a faster, sharper and more stable red chromogen. The newly formulated Warp Red is quick and sensitive, similar to DAB, providing an accelerated protocol and consistent staining quality. The sharp and intense red stain creates superior contrast for Multiplex IHC™. These advanced features of Warp Red result in a faster turnaround time, greater staining consistency and improved flexibility.
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| Vulcan Fast Red Kit 2 | FR805 | Fast Red Chromogen Kit | MP-FR805 |
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Vulcan Fast Red is a widely used chromogen for immunohistochemical staining. When in the presence of alkaline phosphatase (AP) enzyme, Vulcan Fast Red produces a bright fuchsin-red precipitate that is insoluble in organic solvents and can be coverslipped with a permanent mounting media. This product is available in a stable two-component system, consisting of Vulcan Fast Red Chromogen and Buffer. |
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| Ferangi Blue™ Chromogen Kit 2 | FB813 | Blue Chromogen Kit | MP-813 |
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Ferangi Blue™ can be coverslipped with a non xylene or toluene-based permanent mounting media.  This chromogen produces a bright royal blue precipitate in the presence of alkaline phosphatase enzyme. Not for use with PBS. |
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